Fig. 2

ADPRHL deficiency decreases FA formation. A Western blot analysis of β1-integrin and p-FAK in WT-CMs and KO-CMs at day 20. B and C Quantification of β1-integrin and FAK normalized by GAPDH in WT-CMs and KO-CMs at day 20. D Immunofluorescence staining of WT-CMs and KO-CMs for paxillin. Scale bar, 25 μm. E Western blot analysis of N-cadherin and Cx43 in WT-CMs and KO-CMs at day 20. F and G Quantification of N-cadherin and Cx43 in WT-CMs and KO-CMs at day 20. H Immunofluorescence staining of WT-CMs and KO-CMs for Cx43. Scale bar, 7.5 μm. Results are presented as means ± S.E.M. of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; unpaired two-sided Student’s t test