Fig. 5

Inhibition of the ROCK pathway rescues the cell adhesion phenotype of KO-CMs. (A, B and C) Quantification q-PCR analysis ROCK1, ROCK2 and FAK normalized by GAPDH in WT-CMs and KO-CMs at day 20. (D) Adherence of WT-CMs, KO-CMs and KO-CMs with drug treatment after re-seeding on day 15. (E) Quantification of cells adherent relative count. (F) Western blot analysis of N-cadherin and p-FAK in WT-CMs, KO-CMs and KO-CMs treated with Blebbistatin and TZV at day 20. (G) Western blot analysis of Integrin-β1, Cx43, ROCK1 and ROCK2 in WT-CMs, KO-CMs and KO-CMs treated with Blebbistatin at day 20.Results are presented as means ± S.E.M. of three independent experiments. **P < 0.01; ***P < 0.001; unpaired two-sided Student’s t test