Fig. 2

a H4 cells expressing mCherry, or MetRS^L274G and eGFP were cultured separately or on the same surface. Scale bars are 400 µm. Following ANL or vehicle treatment cells were lysed and subjected to BONCAT and affinity purification pipelines. b Silver stain reveals retention of proteins exclusively from MetRS^L274G-expressing cells that received ANL supplementation. c WB showed specificity of retention of GFP from MetRS^L274G-expressing cells receiving ANL, and elimination of RFP from WT cells. Uncropped blots are available in Additional file 2: Fig. S2