Fig. 4

iPSC cells were stably transfected with MetRS^L274G and cultured in 4 mM ANL-containing media. a FUNCAT labeling with Cy5-DBCO shows labeling of translated proteins exclusively in MetRS^L274G-expressing cells. Scale bars are 50 µm. b G-band karyotyping of edited cells shows no induction of chromosomal abnormalities. c To demonstrate persisting pluripotency, cells were differentiated to ectoderm, mesoderm, and endoderm germ layers and labeled with Otx217, brachyury, and SOX17, respectively. Scale bars are 100 µm. d Continued stemness was validated by staining MetRS^L274G-expressing iPSCs with SSEA4 and Oct-4. Scale bars are 50 µm