Skip to main content
Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: A mutant methionyl-tRNA synthetase-based toolkit to assess induced-mesenchymal stromal cell secretome in mixed-culture disease models

Fig. 5

MetRSL274G-expressing iPSCs were differentiated to iMSCs. iMSC identity was validated by a expression of CD73, CD90, and CD105, and lack of expression of CD45, CD34, CD11b, CD79a, and HLA-DR (n = 3), and b multipotency demonstrated by differentiation to adipocytes (mFABP4), osteoblasts (hOsteocalcin), and chondrocytes (Alcian Blue 8GX). c Reverse transcription PCR with species-specific Taqman probe sets was used to confirm continued expression of MetRSL274 (Mars) in iMSCs following differentiation and expansion. Uncropped agarose gel is shown in Additional file 5: Fig. 5. d Functionality of MetRSL274G was confirmed by FUNCAT labeling with Cy5-DBCO in cells cultured in ANL-supplemented media

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512