Table 1 Antibodies used in this manuscript

Hoechst 33,342

Invitrogen (H3570)

1:10,000 (Immunocytochemistry (ICC))

Alpha-Tubulin (mouse)

Millipore-Sigma (T9026)

1:500 (ICC)

Green Fluorescent Protein (rabbit)

Proteintech (50430-2-AP)

1:2,000 (Western blot (WB))

Red Fluorescent Protein [6G6] (mouse)

Chromotek (6g6)

1:1,000 (WB)

α-synuclein [MFJR1] (rabbit)

Abcam (ab138501)

1:2,000 (WB)

Otx217 (goat)

R&D Systems (967331), from StemXVivo Ectoderm Kit (SC031B)

1 µg/100µL (ICC)

Brachyury (goat)

R&D Systems (967332), from StemXVivo Mesoderm Kit (SC030B)

1 µg/100µL (ICC)

Sox17 (goat)

R&D Systems (967330), from StemXVivo Endoderm Kit (SCO19B)

1 µg/100µL (ICC)

SSEA4

Cell Signaling (#4755)

1:500 (ICC)

Oct-4

Cell Signaling (#2750)

1:200 (ICC)

CD73 Monoclonal Antibody (AD2), APC

eBioscience (17-0739-41)

1:20 (Flow cytometry (FC))

Brilliant Violet 421™ anti-human CD90 (Thy1) Antibody

Biolegend (328121)

1:20 (FC)

CD105 (Endoglin) Monoclonal Antibody (SN6), PE

eBioscience (12-1057-41)

1:20 (FC)

CD45 Monoclonal Antibody (HI30), APC

eBioscience (17-0459-41)

1:20 (FC)

CD34 Monoclonal Antibody (4H11), APC

eBioscience (17-0349-41)

1:20 (FC)

CD11b Monoclonal Antibody (ICRF44), APC

eBioscience (17-0118-41)

1:20 (FC)

CD79a Monoclonal Antibody (HM47), PE

eBioscience (12-0792-41)

1:20 (FC)

Brilliant Violet 421™ anti-human HLA-DR Antibody

Biolegend (307635)

1:20 (FC)

Sytox Green

Invitrogen (S34860)

30 nM (1:1,000) (FC)

mFABP4 (goat)

R&D Systems (967799), from Human Mesenchymal Stem Cell Functional Identification Kit (SC006)

10 µg/mL (ICC)

hOsteocalcin (goat)

R&D Systems (967801), from Human Mesenchymal Stem Cell Functional Identification Kit (SC006)

10 µg/mL (ICC)

Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568

Invitrogen (A-11004)

1:500 (ICC)

IRDye^® 800CW Goat anti-Rabbit IgG Secondary Antibody

Licor (925-32211)

1:10,000 (WB)

IRDye^® 680RD Goat anti-Mouse IgG Secondary Antibody

Licor (925-68070)

1:10,000 (WB)

FlexAble CoraLite^®488 Antibody Labeling Kit for Rabbit IgG

Proteintech (KFA001)

1µL/0.5 µg Ab (WB)

Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 555

Invitrogen (A-32727)

1:500 (ICC)

Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647

Invitrogen (A-21245)

1:500 (ICC)

Donkey anti-Goat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488

Invitrogen (A-11055)

1:1,000 ICC

Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568

Invitrogen (A-10037)

1:1,000 ICC