Fig. 3

The ADSC secretome suppresses JAK-STAT and AKT signaling pathway by upregulation of regulator CIS. IL-2-stimulated NK cells were treated with or without 150 μg/ml ADSC secretome for 36 h. Unstimulated NK cells were also cultured for negative control. Immunoblot analysis of A STAT5, AKT, ERK, B JAK1, JAK3, C SHP1, SHP2, SHIP1, and D CIS expressions in NK-92 cells. Full-length blots were presented in Additional file 5: Figure S3 to S6. In the groups A, B, and C, the p- prefix denotes a phosphorylated protein. Error bars represent mean ± SEM of three to four independent experiments. Statistical tests were determined by Student’s paired two-tailed t-test; not significant (ns), *P < 0.05, **P < 0.01, ***P < 0.001