Fig. 3

Global transcriptome analysis reveals suppression of fibroblast and induction of CM precursor signatures in iCMPs. a Principal component analysis of gene expression data generated by RNA sequencing from three sample groups: (1) purified iCMPs; (2) starting cardiac fibroblasts (CFs); and (3) adult heart (n = 2 for each sample group). The 2D principal component analysis plot presents the intra- and intergroup relationships by showing the first two principal components (PCs) that account for the majority (98%) of variation between samples. b K-means clustering and functional overrepresentation analysis of significant differentially expressed genes. Results are depicted as heatmap with accompanying table listing four gene ontology (GO) terms, selected from the top 40 terms with significant gene enrichment, for each of the six k-means clusters. c Analysis of selected marker genes associated with CM development, CM identity, proliferation, fibroblast identity, as well as related and competing cell lineages. Results are depicted as heatmaps. Heatmap columns represent replicate samples, heatmap rows represent single genes, the Z-score color scale indicates relative gene expression levels for each row (magenta, higher expression, Z-score > 0; cyan, lower expression, Z-score < 0; dark magenta, outside the defined range) Figure 4