Fig. 6
Proteomic analysis of iCMP and CF secretomes by mass spectrometry reveals distinct quantitative and qualitative paracrine expression profiles a Number of total and unique proteins detected by mass spectrometry in medium supernatants of iCMPs (n = 3) and CFs (n = 3) cultured under normoxic (21% O2, 1 g/l glucose) or ischemic (1% O2, no glucose) conditions for 24 h or 72 h, revealing a superior secretory activity for iCMPs under ischemic conditions. b Venn diagram for extracellular proteins (gene ontology term 0005576 “extracellular region”) of CFs (104 proteins) and iCMPs (140 proteins) cultured under ischemia I for 24–72 h. Single proteins are listed in Additional file 1: Table S3. c Protein interaction networks and functional enrichment analysis for biological processes and cellular components related to cardiac repair for proteins of CFs and iCMPs in b, generated using the STRING database. c Bar graph showing the enriched biological processes and cellular components related to cardiac repair for iCMPs and CFs, the annotated protein count, and the significance of enrichment. The iCMP secretome is significantly enriched with proteins associated with the regulation of cell death, extracellular matrix, and heart development