Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Blockade of TGF-β signalling alleviates human adipose stem cell senescence induced by native ECM in obesity visceral white adipose tissue

Fig. 1

The properties of ECM in vWAT from lean and obese objects were determined. a Representative image of ECM for Sirius Red stain, immunostained with antibody against type I collagen, and fibronectin. The black arrows indicate staining positive area. Histogram showing the difference of the positive area percentage of Sirius Red, type I collagen, and fibronectin in each field from lean hASCs and obese hASCs. The number of fields was 10–14 in each group, respectively. Data are presented as the means ± SEM. Significant difference by unpaired two-tailed paired t test. Scale bars, 25 μm. b Representative image of ECM for immunostained with antibody against type I collagen (green) and CD31 (red) as well as DAPI nuclear staining (blue). The white arrows indicate staining positive area. Scale bars, 75 μm. c Representative image of ECM for immunostained with antibody against TGF-β1 (green) and CD31 (red) and DAPI nuclear staining (blue). The white arrows indicate staining positive area. Scale bars, 75 μm. vWAT, visceral white adipose tissue

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512