Fig. 5

The characterization of the hASC-derived matrix response was determined. a Representative surface topography and microstructure images of hASC-derived matrix derived from lean and obese donors observed by SEM. Scale bars, 5 μm. b Graph showing the distribution of reorientation angle and anisotropy coefficient of the fibres in lean hASC-derived matrix and obese hASC-derived matrix determined by the ImageJ plug-in FibrilTool. The left panel is the distribution of normalized average fibre orientation (direction angle in degrees in the interval − 90–90° with respect to the major fibril direction of the image). The right panel represents anisotropy of fibre arrays in a given region of interest. 0 for no order (purely isotropic arrays) and 1 for perfectly ordered (parallel fibrils). n = 3 different donors. The number of fields for anisotropy was 12 in each group, respectively. c Representative images of lean hASC-derived matrix and obese hASC-derived matrix for immunostained with antibody against type I collagen, fibronectin, and TGF-β1. Histogram showing the difference of the relative intensity of type I collagen, fibronectin, and TGF-β1 in each field. n = 3 different donors from type I collagen and fibronectin, in which > 7 fields were quantified. Insets: The hASC-derived matrix was counterstained with DAPI. Data are presented as the means ± SEM. Scale bars, 50 μm and 75 μm Significant difference by unpaired two-tailed paired t test. A.U., Arbitrary Unit. Fold represents the relative intensity was normalized against lean hASC-derived matrix from each independent experiment. hASC-matrix, hASC-derived matrix