Fig. 7

Mitochondrial function was determined in lean hASCs cultured on different hASC-derived matrix. a Representative images of lean hASCs cultured on different hASC-derived matrix for the mitochondrial mass and membrane potential determined by MitoTracker Green FM (Green) and TMRM (Red), respectively. Histogram showing the difference of the relative intensity of TMRM and MitoTracker Green in each cell from lean hASCs cultured on different hASC-derived matrix. The number of cells was 65–112 in each group, respectively. n = 3 independent experiments from one lean hASC-derived matrix; n = 3 different donors from obese hASC-derived matrix. Scale bars, 25 μm. Fold represents the relative intensity was normalized against lean hASC-derived matrix from each independent experiment. b Kinetic profile of the OCR was measured in lean hASCs cultured on different hASC-derived matrix using a Seahorse XF Cell Mito Stress test. The OCR was normalized against cells from each independent experiment. Black arrows show the times of treatment with Oligo, FCCP, Rot, and AA. Histogram showing the difference of the OCR of the lean hASC for different parameters from after cultured on different hASC-derived matrix. c Representative images of lean hASCs cultured on the anti-TGF-β1 neutralizing antibody and control IgG1-treated obese hASC-derived matrix for the mitochondrial mass and membrane potential determined by MitoTracker Green FM (Green) and TMRM (Red), respectively. Histogram showing the difference of the relative intensity of MitoTracker Green and TMRM in each cell from lean hASCs cultured on the anti-TGF-β1 neutralizing antibody and control IgG1-treated obese hASC-derived matrix. Fold represents the relative intensity was normalized against IgG1 group from each independent experiment. The number of cells was 51–112 in each group, respectively. n = 3 different donors. Scale bars, 25 μm. d Kinetic profile of the OCR was measured in lean hASCs cultured on the anti-TGF-β1 neutralizing antibody-treated obese hASC-derived matrix using a Seahorse XF Cell Mito Stress test. Fold represents the OCR was normalized against IgG1 group from each independent experiment. Black arrows show the times of treatment with Oligo, FCCP, Rot, and AA. Histogram showing the difference of the OCR of the lean hASC for different parameters from after cultured on different hASC-derived matrix. n = 2 different donors. Significant difference by unpaired two-tailed paired t test. Data are presented as the means ± SEM. OCR, oxygen consumption rate; oligo, oligomycin; FCCP, carbonyl cyanide 4-trifluoromethoxy-phenylhydrazone; rot: rotenone; AA, antimycin A; hASC-matrix, hASC-derived matrix; anti-TGF-β1, anti-TGF-β1 neutralizing antibody