Fig. 2

Levels of white blood cell numbers, LSK cells, and plasma soluble P-selectin in G-CSF-treated mice. The experimental outline is shown (A). Peripheral blood was collected before and on day 2 and 4 after G-CSF treatments (250 μg/kg/day, n = 6, two experiments with six mice per group). Normal saline-treated mice (n = 4, two experiments with four mice per group) served as negative controls. White blood cell (WBC) numbers (B), LSK (Lin⁻/Sca-1⁺/c-Kit⁺) cell numbers (C), and plasma sP-sel (D) were detected using hematology analysis, flow cytometry (FC), and ELISA, respectively. Data are reported as mean ± standard deviation (SD). **P < 0.01 compared with the before experiment groups (Before Exp)