Fig. 3

Comparison of LSK cells and P-selectin in peripheral blood between C57BL/6J wild-type and P-selectin-deficiency mice. The experimental outline used to monitor LSK cells and P-selectin in peripheral blood is illustrated (A). C57BL/6J wild-type (WT) mice (n = 7, five experiments with seven mice per group) and P-selectin-deficiency mice (Selp^−/−; n = 6, five experiments with six mice per group) were injected with G-CSF (250 μg/kg/day) for 4 consecutive days. P-selectin-deficiency mice (Selp^−/−; n = 6, five experiments with six mice per group) were injected with WT platelets (1 × 10⁸) once a day, together with the last three doses of G-CSF. LSK cell numbers were detected using flow cytometry (FC) 1 week before and on day 4 after G-CSF treatment. The LSK cell numbers of G-CSF treated-WT mice were set as onefold. Each group’s relative folds of LSK cell numbers are shown (B). The concentrations of P-selectin in either microparticles or soluble forms in the peripheral blood of each group were further characterized using immunoassays (C). Two groups were included in the study: Selp^−/− mice injected with G-CSF (n = 4, two experiments with four mice per group) and Selp^−/− mice injected with G-CSF and WT platelets (n = 4, two experiments with four mice per group). Data are reported as mean ± SD. **P < 0.01 compared with the microparticle groups within each group. ^##P < 0.01 compared with the indicated groups.