Fig. 4

Comparison of LSK cell numbers in the peripheral blood between mice treated with G-CSF, soluble P-selectin, and combined G-CSF and soluble P-selectin. The experimental outline for monitoring LSK cells in the peripheral blood is illustrated (A). C57BL/6J wild-type (WT) mice were injected with 4 consecutive days of G-CSF (250 μg/kg/day, n = 6, three experiments with six mice per group), combined G-CSF (250 μg/kg/day) and sP-sel (0.1 mg/kg, n = 4, one experiment with four mice per group), or three different doses of sP-sel (0.1 mg/kg, n = 6, two experiments with six mice per group; 0.5 mg/kg, n = 4, two experiments with four mice per group; 1.0 mg/kg, n = 6, two experiments with six mice per group). C57BL/6J mice injected with human IgG Fc fragment (1 mg/kg, n = 4, one experiment with four mice per group) served as a control group. The LSK cells in peripheral blood were analyzed and quantified using flow cytometry (FC) (B). Data are reported as mean ± SD. *P < 0.05 and **P < 0.01 compared with the before experiment groups (Before Exp.). ^##P < 0.01 compared with the indicated groups.