Fig. 5

Comparison of P-selectin positive cell percentages in LSK populations between G-CSF- and soluble-P-selectin-treated groups. The experimental procedure for assessing the percentage of P-selectin positive (P-sel⁺) cells in LSK groups is shown (A). C57BL/6J wild-type (WT) mice were injected with either 4 consecutive days of G-CSF (250 μg/kg/day, n = 4, one experiments with four mice per group) or two doses of sP-sel (1 mg/kg, n = 4, one experiments with four mice per group). The LSK cell counts in peripheral blood (B) and the percentages of P-sel⁺ cells within LSK populations (C) were determined and quantified using flow cytometry (FC) 17 h after the final injection. Data are presented as mean ± SD. ^**P < 0.01 compared with the indicated groups