Fig. 1
From: HIF-1α promotes kidney organoid vascularization and applications in disease modeling
Generation of kidney organoid from human induced pluripotent stem cells. A Schematic diagram of kidney organoid differentiation for 18 days, modified from Morizane et al., Takasota et al., and Monteil et al. B Phase contrast images from HiPSC to kidney organoid for 18 days. HiPSCs were differentiated to day 7 to kidney progenitor cells. With FGF9 with heparin treatment, kidney organoids matured at day 18 with rough circles. C The confocal images for glomerular pars and proximal tubule parts for kidney organoid. The images showed the staining analysis of the proximal tubule (LTL, green), podocyte (nephrin, red), and phase contrast images. D The confocal images for proximal tubule (LTL, green) and distal nephrin (CDH1, red). E The confocal images for endothelial cells (CD31, yellow), podocytes (Wilms' Tumor, red), and proximal tubule (LTL, green)