Fig. 3
From: HIF-1α promotes kidney organoid vascularization and applications in disease modeling
Overexpression of HIF-1α in kidney organoids promotes vascularization. A The immunofluorescent result for endothelial networks in kidney organoid. Z-stack confocal analyses were done for kidney organoids (Wilms' Tumor, red; LTL, green) with endothelial networks (CD31, yellow). The data were periphery, center, and merged images of kidney organoid. Five groups of organoids were displayed for control, HIF-1α overexpressing group, DMOG group, HIF-1α with semaxnib group, and HIF-1α with axitinib treated group. Scale bar, 50 μm. B The quantification of immunoreactive signals for CD31 (B), LTL (C), and WT-1 (D). Each dot represents the percentage of immunoreactive signal CD31/LTL/WT-1 positive area to DAPI area in each kidney organoid (n = 5–8 per group, *P < 0.05, **P < 0.01 and ****P < 0.0001 by unpaired t test)