Fig. 4
From: HIF-1α promotes kidney organoid vascularization and applications in disease modeling
The confocal analysis in HIF-1α-overexpressing kidney organoid. A The 3D confocal analysis revealed that transfected HIF-1αkidney organoid positioned glomerular compartments (WT-1, yellow) and tubular parts (LTL, green) packed with endothelial networks (CD31, red). The larger image was HIF-1α transfected group. From left to right in the second line, the four images were controls, DMOG, HIF-1α with semaxanib, and HIF-1α with axitinib treated organoid. B The relative mRNA expression of CD31. C We use Angiotool analysis software to analyze blood vessels in kidney organoids. The vessel percentage area (C), average vessel length (D), total number of junctions and Mean E lacunarity (E), vessel percentage area (F). Each group of samples was displayed. (n = 6–8 per group, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by unpaired t test)