Fig. 5

The α-SMA expression in HIF-1α-overexpressing kidney organoid. A The immunofluorescent result for vascular smooth muscle cells in kidney organoid. The confocal analysis was done for kidney organoid (LTL, orange) with vascular smooth muscle cells (α-SMA, green) and endothelial networks (CD31, red). The five groups of organoids were the control group, HIF-1α overexpressing plasmid group, DMOG group, HIF-1α with semaxnib group, and HIF-1α with axitinib group. Scale bar, 50 μm. B The quantification of immunoreactive signals for α-SMA. Each dot represents the percentage of immunoreactive signal α-SMA positive area to DAPI area in each kidney organoid (n = 4–6 per group, *P < 0.05 by unpaired t test). C The ratio of α-SMA positive area to CD31positive area. (n = 4–6 per group, **P < 0.01 and ***P < 0.001 by unpaired t test). D The ratio of α-SMA to LTL positive area. (n = 4–6 per group, **P < 0.01 and ***P < 0.001 by unpaired t test)