Fig. 6
From: HIF-1α promotes kidney organoid vascularization and applications in disease modeling
The role of HIF-1α in cisplatin treated kidney organoid. A The confocal analysis revealed the immunofluorescent signals of cleaved caspase 3 (red) in kidney organoid (nephrin, yellow, LTL, green). The four groups of organoids were control organoids, cisplatin treated organoids, cisplatin with additional HIF-1α treated organoids, and HIF-1α overexpressing organoids. Scale bar, 50 μm. E The confocal analysis revealed the immunofluorescent signals of cleaved caspase 3 (red) and CD31 (yellow) in kidney organoid (LTL, green). Scale bar, 50 μm. B-D and F The quantification of immunoreactive signals for Cleaved caspase 3 (B), Nephrin (C), LTL (D), CD31 (F). Each dot represents the percentage of Cleaved caspase 3 (B), Nephrin (C), LTL (D), CD31 (F) positive area to DAPI area (n = 4–5 per group, *P < 0.05 by unpaired t test)