Fig. 7
From: HIF-1α promotes kidney organoid vascularization and applications in disease modeling
The SOD2 or HO-1 expression in cisplatin treated kindey organoid. A The confocal analysis revealed the immunofluorescent signals of SOD2 (red) in kidney organoid (nephrin, yellow; LTL, green). Scale bar, 50 μm. C. The relative SOD2 mRNA expression. B and D. The quantification of immunoreactive signals for SOD2 (B)/ HO-1 (D). Each dot represents the percentage of immunofluorescent signal SOD2 (B)/ HO-1(D) positive area/DAPI area in each kidney organoid (n = 4–7 per group, ***P < 0.001 and ****P < 0.0001 by unpaired t test). E The immunofluorescent analysis showed the signals of HO-1(red) in kidney organoid (nephrin, yellow, LTL, green). The three groups were CTR, cisplatin and cisplatin with HIF-1α.Scale bar, 50 μm