Fig. 5

The impact of macrophage depletion on ESC–MSC-EVs therapy in severe skeletal muscle injury caused by ACS. A Representative immunohistochemistry images (200x) of TA muscle sections stained with CD68 at 3 d after ACS. Scale bar: 100 μm. B Quantitative assessment of cells positive for CD68 (n = 6). C Representative images of CD206, CD68, and CD86 expression levels of skeletal muscle by Western blotting at 3 d after ACS. D–F Quantitative analysis expression of CD206, CD68, and CD86 (n = 6). G Representative images (200×) of HE staining of TA muscle sections at 3 d after ACS. Scale bar: 100 μm. H Percentage of injured myofibers (n = 6). I Representative Western blotting images of iNOS and Cleaved caspase 3 expression levels of skeletal muscle at 3 d. J–K Quantitative analysis expression of iNOS and Cleaved caspase 3 (n = 6). L Serum CK levels at 3 d (n = 6). M–O The mRNA expression of TNF-α, IL-6, and IL-10 in skeletal muscle tissue at 3 d after ACS (n = 6). Data are presented as the mean ± SD. *P < 0.05 versus Sham + vehicle group and ^#P < 0.05 versus ACS + vehicle group. The blots of Additional file 2: Fig. S5C and I were all cropped, and full-length blots were presented in Additional file 2: Figs. S4 and S5