Fig. 6

Effect of inflammation on Gene Expression of ADMSCs. A Correlation of expression patterns in ADMSCs between high (n = 3; male = 2, female = 1) and low (n = 3; male = 1, female = 2) inflammation level. Correlation matrix of the entire dataset. The analysis was performed by comparing the values of the entire transcriptome in all six samples. Correlation analysis was performed using R software. B Venn chart for gene expression comparisons between High (blue) and Low (green) inflammation level. C Heat map of 106 differentially expressed genes in ADMSCs from High versus Low inflammation level. Each row represents a gene. Expression values for each gene are scaled across each row as z-scores. n = 3 for each group. D KEGG pathway database was used for analyzing differentially expressed genes. Seven signaling pathways were significantly enriched in ADMSCs from the high inflammation tissue, and two of the pathways (red box) were involved in regulating the adipogenic differentiation of MSCs. E, F Gene Ontology (GO) enrichment analysis of 106 differentially expressed genes. Bar graph shows fold enrichment for the top five GO Biological Process terms enriched at p ≤ 0.05. MSC adipogenic differentiation-related pathways were significantly enriched in High-MSC group cells (blue box). GO-MF (e), GO Molecular function; GO-BP (f), GO Biological process. G Differentially expressed marker genes related to the MSC trilineage differentiation. The gene expression levels are estimated in terms of “transcripts per million”