Fig. 3

Determination of differentiation potential of LP and HP pMSCs grown in FM and XM. a–c Fluorescence microscopic images and fluorescence intensity of the derivatives stained with CEBP, COL2, and OCN antibodies representing chondrogenic, osteogenic, and adipogenic lineages, respectively. d Quantification of fluorescent intensity of immunostained derivatives of pMSCs performed in triplicate. Scale bars represent 400 μm (magnification: 40×). e Expression of adipogenic (CEBPβ, FABP4, and PPARγ), chondrogenic (SOX9, ACAN, and COL2), and osteogenic (COL1, RUNX2, OPN, and OCN) genes in the differentiated derivatives. Gene expression was normalized to GAPDH and ACTIN, and error bars represent the standard deviations of the triplicate measurements. HP pMSCs grown in FM showed a greater propensity to differentiate toward adipogenic lineage. Any results showing **p ≤ 0.01 and *p ≤ 0.05 were deemed statistically significant