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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Regulation of self-renewal and senescence in primitive mesenchymal stem cells by Wnt and TGFβ signaling

Fig. 5

Nucleosome mapping and genome browser shots showing nucleosome occupancy determined by Mnase-seq and IGV analysis a–c Nucleosome mapping showing cell cycle, DNA replication, and senescence genes, respectively. d–f Wnt pathway genes (FZD1, LRP6, and Wnt2B), g–j VEGF/PDGF pathway genes (VEGFA, FLT1, PDGFC, and PDGFRA), k cell cycle gene (PSMD7), L–M Self-renewal and DNA replication genes (MYC and PCNA), and n–o senescence (CXCL8 and CDKN2A). Data showed less nucleosome occupancies in pMSCs cultured in XM compared to FM in cell cycle and DNA replication genes. In contrast, more nucleosome occupancies in pMSCs cultured in XM than FM were found in senescence genes. IGV mapping showed that FZD1, LRP6, Wnt2B, VEGFA, FLT1, PDGFC, PDGFRA, PSMD7, MYC, and PCNA genes had less nucleosome occupancies around the promoters, suggesting the higher expression in pMSCs grown in XM than FM, whereas CXCL8 and CDKN2A had higher nucleosome occupancies around the promoters, suggesting their lower expression in pMSCs grown in XM compared to FM. LP and HP pMSCs grown in FM (blue and green, respectively), LP and HP pMSCs grown in XM (orange and purple, respectively)

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Stem Cell Research & Therapy

ISSN: 1757-6512