Fig. 7

Effect of Wnt and TGFβ agonist and antagonist on pMSCs cultured in FM and XM. a, b The proliferation of pMSCs cultured in the 10 mM Wnt agonist, LiCl, and 500 nM Wnt antagonist, TFA, determined by direct counts and using BrdU proliferation assay. c Relative expression of selected genes involved in self-renewal in pMSCs cultured in media containing LiCl or TFA compared to the control. d, e The proliferation of cells cultured in 0.25 mM TGFβ agonist, BMPSB4, and 0.125 mM TGFβ antagonist, Asiaticoside, determined by direct counts and using BrdU proliferation assay. f Relative expression of selected genes involved in TGFβ and self-renewal in cells supplemented with BMPSB4 and Asiaticoside compared to the control Gene expression was normalized to GAPDH and ACTIN, and error bars represent the standard deviations of the triplicate measurements. Significance was measured in comparison of the control. Any results showing **p ≤ 0.01 and *p ≤ 0.05 were deemed statistically significant