Fig. 6

hWJ-MSCs grown on hADM scaffold could differentiate into epithelial-like cells. Absence of filaggrin on day 14 in hWJ-MSCs grown on TCP with and without epithelial medium (A and B). Filaggrin production in hWJ-MSCs seeded on hADM and air–liquid system (C) and treated with epithelial induction medium (D). Involucrin synthesis in hWJ-MSCs seeded on TCP (E and F), in the construct with hPL-supplemented DMEM (G) and cultured with epithelial induction medium (H). Plakoglobin absence in the monolayer culture (I and J), and Plakoglobin synthesis in the construct cultured with hPL-supplemented DMEM or epithelial medium, respectively (K and L). Scale bar = 100 μm. n = 3. TCP tissue culture plate, KGM keratinocytes growth medium or epithelial induction medium