Fig. 2

Assessment of alkaline phosphatase (ALP) expression in iPSC lines prior and throughout mesodermal and hematopoietic differentiation. A-L Representative images of 3 replicates display ALP-stained colonies and cell-forming complexes of a PBMC-derived cell line (A-F) and a fibroblast-derived cell line (G-L), showing high expression of ALP while expanding (A, B, G, H). Following mesodermal and hematopoietic differentiation according to either the embryoid body-based (C, D, I, J) or monolayer-based protocol (E, F, K,L), cell-forming complexes retained ALP expression, especially in centrally located cells (solid arrows). An abundance of ALP-negative cells can be observed following hematopoietic differentiation (empty arrows). Scale bar = 500 µm