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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins

Fig. 4

Assessment of hematopoietic cells using flow cytometry. A, D Undifferentiated iPSCs were used as a reference for marker expression. B, C, E, F Hematopoietic cells differentiated according to the embryoid body-based (EB) protocol show a higher expression of later hematopoietic markers CD43 and CD45 (B, E) in comparison with monolayer-based (MB) differentiated cells (C, F). Additionally, the monocyte markers CD14 and CD11b were elevated in the EB group (B, E). No differences in RANK expression could be observed between either differentiation protocol

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