Fig. 1

MSCs induced the expression of CD32b on CD4⁺ T cells. A Images showing the morphology of mouse-derived MSCs (magnification 40 ×). B Flow cytometric analysis of the MSC surface markers CD29, CD34, CD44, CD45, and SCA-1. C Flow cytometric analysis of the GFP-positive rates of MSCs before and after puromycin screening. D ELISA of sFgl2 concentrations (ng/mL) in the supernatants of different kinds of MSCs. E Flow cytometric analysis of CD32b expression levels on CD4⁺ T cells cocultured with modified or unmodified MSCs, n = 3. F Proportions of CD32b-positive CD4⁺ T cells in each group. The differences between groups were calculated using ANOVA. ***: P < 0.001. Abbreviations: MSCs, mesenchymal stem cells; sFgl2, soluble fibronectin-like protein 2; WT-MSCs, wild-type MSCs; NC-MSCs, negative control MSCs, GFP, green fluorescent protein