Fig. 2

In vitro, sFgl2-MSCs increased the proportion of Tregs and reduced the proportion of Th17 and Th1 cells. A Dot plots showing Tregs (CD4⁺CD25⁺Foxp3⁺) among CD4⁺ T cells after coculture for 72 h. B Dot plots showing Th17 cells among CD4⁺ T cells after coculture for 72 h. C Dot plots showing Th1 cells among CD4⁺ T cells after coculture for 72 h. D Proportions of Tregs (CD4⁺CD25⁺Foxp3⁺) in each group, n = 3, #: rFgl2 versus control, P < 0.05. E Proportions of Th17 cells (CD4⁺IL-17A⁺) in each group, n = 3, #: rFgl2 versus control, P < 0.001. F Proportions of Th1 cells (CD4⁺IFN-γ⁺) in each group, n = 3, #: rFgl2 versus control, P < 0.001. G Levels of p-SHP2 in each group. H Levels of p-SMAD2/3 in each group. Blots were cropped, and the original blots are presented in Additional file 1: Fig. S1 and Additional file 2: Fig. S2. The differences between groups were calculated using ANOVA. ***: P < 0.001. Abbreviations: MSCs, mesenchymal stem cells; sFgl2, soluble fibronectin-like protein 2; WT-MSCs, wild-type MSCs; NC-MSCs, negative control MSCs