Fig. 2
From: Bone marrow from periacetabular osteotomies as a novel source for human mesenchymal stromal cells
Post-thaw viability, proliferation capacity, metabolic activity and senescence of BM-MSCs isolated from bone samples obtained during PAO and THA. A Cell viability after thawing of cells. B Cell recovery after thawing. C Metabolic activity of thawed cells 24 h after seeding relative to cell number. D Cell numbers determined by DNA quantification at days 0 (24 h after seeding), 4 and 7. E Population doublings of cells at days 4 and 7. F Metabolic activity relative to cell number at days 0 (24 h after seeding), 4 and 7. G Representative fluorescence images of BM-MSCs from cell culture passage 4 and 5 stained for senescence-associated beta galactosidase. Green, beta galactosidase; blue, nuclei. H Intensities of the beta galactosidase signals. I Fold change of the beta galactosidase signals. [normally distributed data: mean ± SD; non-normally distributed data: median with IQR; levels of significance: *p < 0.05] Abbreviation: β-gal, beta galactosidase