Fig. 1
Therapeutic effects of SF-MSCs on CIN model mice. A Establishment of CIN model mice and treatment groups. CIN model mice were divided into three groups after irradiation: PBS: PBS injection (100 µL); MSC: MSCs injection (1.0 × 105 cells in 100 µL PBS); and SF-MSC: SF-MSCs injection (1.0 × 105 cells in 100 µL PBS). B Serum levels of blood urea nitrogen (BUN) and creatinine (Cr) (n = 5 in each group). C Representative hematoxylin–eosin (HE) staining images and tubular injury score of the kidney. Ten high magnification fields (×200) of the renal cortex and cortico-medullary junction were randomly selected from each mouse (n = 3 in sham, n = 5 in other groups). The extent of degeneration and detachment of tubular cells were scored as described in Methods. All assessments were done by two investigators blinded to experimental conditions. Scale bar: 100 µm. Data are means ± S.D. #P < 0.01 (one-way ANOVA followed by Tukey–Kramer’s post-hoc test)