Fig. 2

Effects of MSCs and SF-MSCs on DNA damage and apoptosis in the CIN model mouse kidney. A Immunoblotting analysis of γH2AX in kidney tissue from CIN model mice sacrificed at 24 h after tail vein injection (n = 5 in each group). GAPDH was used as a loading control. Full-length blots are presented in Additional file 1: Fig. 1a. B Representative immunostaining images for γH2AX in the kidney. Scale bar: 100 µm. Graph shows the average number of γH2AX-positive cells (n = 3 in sham, n = 5 in other groups). Ten high magnification fields (×200) of the renal cortex and cortico-medullary junction were randomly selected, and the average number of γH2AX-positive cells per field was calculated. C Immunoblotting analysis of cleaved caspase-3 in kidney tissue from CIN model mice sacrificed at 24 h after tail vein injection (n = 5 in each group). GAPDH was used as a loading control. Full-length blots are presented in Additional file 2: Fig. 1b. D Representative images for TdT-mediated dUTP nick end labeling (TUNEL) staining in the kidney. Scale bar: 100 µm. Graph shows the average number of TUNEL-positive cells (n = 3 in sham, n = 5 in other groups). Data are means ± S.D. ^#P < 0.01, *P < 0.05 (one-way ANOVA followed by Tukey–Kramer’s post-hoc test)