Fig. 6

Knockdown of EGF in SF-MSCs reduces the anti-apoptotic effect in CIN model mice. A Immunoblotting analysis of γH2AX in kidney tissue from CIN model mice sacrificed at 24 h after tail vein injection (n = 5 in each group). GAPDH was used as a loading control. Full-length blots are presented in Additional file 1: Fig. 1d. B Representative immunostaining of γH2AX in kidney from each group of mice. Scale bar: 100 µm. Graph shows the average number of γH2AX-positive cells (n = 3 in sham, n = 5 in other groups). Ten high magnification fields (×200) of the renal cortex and cortico-medullary junction were randomly selected, and the average number of γH2AX positive cells per field was calculated. C Immunoblotting analysis of cleaved caspase-3 in kidney tissue from CIN model mice sacrificed at 24 h after tail vein injection. GAPDH was used as a loading control (n = 5 in each group). Full-length blots are presented in Additional file 1: Fig. 1e. D Representative image of TdT-mediated dUTP nick end labeling (TUNEL) staining in the kidney from each group of mice. Scale bar: 100 µm. Graph shows the average number of TUNEL-positive cells (n = 3 in sham, n = 5 in other groups). Ten high magnification fields (×200) of the renal cortex and cortico-medullary junction were randomly selected, and the average number of TUNEL positive cells per 1 field was calculated. E Immunoblotting analysis of cleaved caspase-3 in irradiated HEK-293 cells incubated for 24 h with SF-MSC-CM or SF-MSC-CM with 10 µM erlotinib (n = 5 in each group). α-Tubulin was used as a loading control. Full length blots are presented in Additional file 1: Fig. 1f. Data are means ± S.D. ^#P < 0.01, *P < 0.05 (one-way ANOVA followed by Tukey–Kramer’s post-hoc test)