Table 2 List of the molecular functions with statistically significant over-/under-representation in the group of the 157 common proteins of hAMSCs secretome pools 1–4
Molecular function | Homo sapiens REFLIST (20,589) | Client text box input (157) | Client text box input (expected) | Client text box input (over/ under) | Client text box input (fold Enrichment) | Client text box input (raw P-value) | Client text box input (FDR) |
|---|---|---|---|---|---|---|---|
Protein disulfide isomerase activity (GO:0003756) | 10 | 5 | 0.08 |  +  | 65.57 | 6.58E-08 | 3.59E-05 |
Collagen binding (GO:0005518) | 8 | 2 | 0.06 |  +  | 32.79 | 2.46E-03 | 2.44E-02 |
Extracellular matrix binding (GO:0050840) | 13 | 3 | 0.1 |  +  | 30.26 | 2.21E-04 | 4.03E-03 |
Disulfide oxidoreductase activity (GO:0015036) | 18 | 4 | 0.14 |  +  | 29.14 | 2.08E-05 | 7.56E-04 |
Protein-disulfide reductase activity (GO:0015035) | 15 | 3 | 0.11 |  +  | 26.23 | 3.19E-04 | 4.84E-03 |
Misfolded protein binding (GO:0051787) | 16 | 3 | 0.12 |  +  | 24.59 | 3.77E-04 | 5.42E-03 |
Aminopeptidase activity (GO:0004177) | 11 | 2 | 0.08 |  +  | 23.84 | 4.20E-03 | 3.76E-02 |
Oxidoreductase activity, acting on a sulfur group of donors (GO:0016667) | 26 | 4 | 0.2 |  +  | 20.18 | 7.42E-05 | 1.84E-03 |
ATP binding (GO:0005524) | 41 | 5 | 0.31 |  +  | 15.99 | 2.48E-05 | 7.98E-04 |
Isomerase activity (GO:0016853) | 76 | 8 | 0.58 |  +  | 13.8 | 2.41E-07 | 6.58E-05 |
Serine-type endopeptidase inhibitor activity (GO:0004867) | 40 | 4 | 0.31 |  +  | 13.11 | 3.39E-04 | 5.00E-03 |
Endopeptidase inhibitor activity (GO:0004866) | 66 | 6 | 0.5 |  +  | 11.92 | 1.77E-05 | 7.42E-04 |
Heat shock protein binding (GO:0031072) | 33 | 3 | 0.25 |  +  | 11.92 | 2.53E-03 | 2.46E-02 |
Peptidase inhibitor activity (GO:0030414) | 66 | 6 | 0.5 |  +  | 11.92 | 1.77E-05 | 6.89E-04 |
Endopeptidase regulator activity (GO:0061135) | 70 | 6 | 0.53 |  +  | 11.24 | 2.41E-05 | 8.23E-04 |
Peptidase regulator activity (GO:0061134) | 72 | 6 | 0.55 |  +  | 10.93 | 2.80E-05 | 8.48E-04 |
Protease binding (GO:0002020) | 78 | 6 | 0.59 |  +  | 10.09 | 4.26E-05 | 1.11E-03 |
Unfolded protein binding (GO:0051082) | 69 | 5 | 0.53 |  +  | 9.5 | 2.46E-04 | 4.34E-03 |
Actin filament binding (GO:0051015) | 135 | 9 | 1.03 |  +  | 8.74 | 1.54E-06 | 1.40E-04 |
Enzyme inhibitor activity (GO:0004857) | 115 | 7 | 0.88 |  +  | 7.98 | 4.02E-05 | 1.16E-03 |
Metallopeptidase activity (GO:0008237) | 116 | 7 | 0.88 |  +  | 7.91 | 4.24E-05 | 1.16E-03 |
Metalloendopeptidase activity (GO:0004222) | 84 | 5 | 0.64 |  +  | 7.81 | 5.80E-04 | 7.19E-03 |
Serine-type endopeptidase activity (GO:0004252) | 103 | 6 | 0.79 |  +  | 7.64 | 1.82E-04 | 3.68E-03 |
Serine hydrolase activity (GO:0017171) | 107 | 6 | 0.82 |  +  | 7.35 | 2.21E-04 | 4.32E-03 |
Serine-type peptidase activity (GO:0008236) | 107 | 6 | 0.82 |  +  | 7.35 | 2.21E-04 | 4.17E-03 |
Actin binding (GO:0003779) | 199 | 11 | 1.52 |  +  | 7.25 | 6.06E-07 | 6.61E-05 |
Calcium ion binding (GO:0005509) | 168 | 9 | 1.28 |  +  | 7.03 | 8.38E-06 | 5.08E-04 |
Cell adhesion molecule binding (GO:0050839) | 141 | 6 | 1.08 |  +  | 5.58 | 8.98E-04 | 1.07E-02 |
Metal ion binding (GO:0046872) | 268 | 11 | 2.04 |  +  | 5.38 | 9.44E-06 | 5.15E-04 |
Protein-containing complex binding (GO:0044877) | 394 | 15 | 3 |  +  | 4.99 | 5.34E-07 | 7.28E-05 |
Endopeptidase activity (GO:0004175) | 324 | 12 | 2.47 |  +  | 4.86 | 9.99E-06 | 4.96E-04 |
Cation binding (GO:0043169) | 325 | 12 | 2.48 |  +  | 4.84 | 1.03E-05 | 4.68E-04 |
Peptidase activity (GO:0008233) | 433 | 15 | 3.3 |  +  | 4.54 | 1.66E-06 | 1.30E-04 |
Cytoskeletal protein binding (GO:0008092) | 411 | 14 | 3.13 |  +  | 4.47 | 4.52E-06 | 3.09E-04 |
Purine ribonucleotide binding (GO:0032555) | 271 | 8 | 2.07 |  +  | 3.87 | 1.32E-03 | 1.47E-02 |
Carbohydrate derivative binding (GO:0097367) | 345 | 10 | 2.63 |  +  | 3.8 | 3.84E-04 | 5.38E-03 |
Ribonucleotide binding (GO:0032553) | 278 | 8 | 2.12 |  +  | 3.77 | 1.54E-03 | 1.68E-02 |
Ion binding (GO:0043167) | 741 | 21 | 5.65 |  +  | 3.72 | 3.17E-07 | 5.77E-05 |
Purine nucleotide binding (GO:0017076) | 288 | 8 | 2.2 |  +  | 3.64 | 1.91E-03 | 2.04E-02 |
Nucleoside phosphate binding (GO:1,901,265) | 330 | 8 | 2.52 |  +  | 3.18 | 4.29E-03 | 3.78E-02 |
Nucleotide binding (GO:0000166) | 330 | 8 | 2.52 |  +  | 3.18 | 4.29E-03 | 3.72E-02 |
Oxidoreductase activity (GO:0016491) | 431 | 10 | 3.29 |  +  | 3.04 | 1.99E-03 | 2.09E-02 |
Anion binding (GO:0043168) | 437 | 10 | 3.33 |  +  | 3 | 2.19E-03 | 2.26E-02 |
Small molecule binding (GO:0036094) | 440 | 10 | 3.36 |  +  | 2.98 | 2.30E-03 | 2.33E-02 |
Enzyme regulator activity (GO:0030234) | 472 | 10 | 3.6 |  +  | 2.78 | 3.75E-03 | 3.42E-02 |
Catalytic activity, acting on a protein (GO:0140096) | 1473 | 25 | 11.23 |  +  | 2.23 | 2.50E-04 | 4.27E-03 |
Hydrolase activity (GO:0016787) | 1739 | 26 | 13.26 |  +  | 1.96 | 1.29E-03 | 1.46E-02 |
Protein binding (GO:0005515) | 2812 | 40 | 21.44 |  +  | 1.87 | 9.45E-05 | 2.15E-03 |
Catalytic activity (GO:0003824) | 3916 | 50 | 29.86 |  +  | 1.67 | 1.38E-04 | 3.02E-03 |
Nucleic acid binding (GO:0003676) | 1974 | 3 | 15.05 | − | 0.2 | 2.95E-04 | 4.60E-03 |
Molecular transducer activity (GO:0060089) | 1085 | 1 | 8.27 | − | 0.12 | 3.51E-03 | 3.31E-02 |
Signaling receptor activity (GO:0038023) | 1085 | 1 | 8.27 | − | 0.12 | 3.51E-03 | 3.25E-02 |
DNA binding (GO:0003677) | 1361 | 1 | 10.38 | − | 0.1 | 5.15E-04 | 6.53E-03 |
Transporter activity (GO:0005215) | 770 | 0 | 5.87 | − |  < 0.01 | 4.76E-03 | 4.06E-02 |
Transcription regulator activity (GO:0140110) | 1265 | 0 | 9.65 | − |  < 0.01 | 7.79E-05 | 1.85E-03 |
cis-regulatory region sequence-specific DNA binding (GO:0000987) | 810 | 0 | 6.18 | − |  < 0.01 | 3.15E-03 | 3.02E-02 |
DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981) | 984 | 0 | 7.5 | − |  < 0.01 | 9.51E-04 | 1.10E-02 |
RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978) | 798 | 0 | 6.09 | − |  < 0.01 | 5.09E-03 | 4.27E-02 |
RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977) | 1059 | 0 | 8.08 | − |  < 0.01 | 4.09E-04 | 5.59E-03 |
Transcription cis-regulatory region binding (GO:0000976) | 1099 | 0 | 8.38 | − |  < 0.01 | 4.33E-04 | 5.77E-03 |
Double-stranded DNA binding (GO:0003690) | 1164 | 0 | 8.88 | − |  < 0.01 | 1.79E-04 | 3.76E-03 |
Sequence-specific DNA binding (GO:0043565) | 1148 | 0 | 8.75 | − |  < 0.01 | 2.85E-04 | 4.57E-03 |
DNA-binding transcription factor activity (GO:0003700) | 1054 | 0 | 8.04 | − |  < 0.01 | 6.67E-04 | 8.09E-03 |
Sequence-specific double-stranded DNA binding (GO:1,990,837) | 1117 | 0 | 8.52 | − |  < 0.01 | 2.71E-04 | 4.48E-03 |
Transcription regulatory region nucleic acid binding (GO:0001067) | 1099 | 0 | 8.38 | − |  < 0.01 | 4.33E-04 | 5.63E-03 |