Table 1 Reagents information and concentration
Antibody and regents | Vendor | Catalog No | Dilution or concentration |
|---|---|---|---|
Flow cytometry | Â | Â | Â |
APC anti-human CD4 | Biolegend, California, USA | 300,514 | 1:100 |
BD Stem flow hMSC analysis Kit | BD Biosciences, California, USA | 562,245 | According to the manufacture instruction |
CellTrace CFSE cell proliferation Kit | Invitrogen, California, USA | C34554 | 5 μM |
Annexin V-FITC/PI apoptosis kit | MULTI SCIENCES, Zhejiang, China | AP 101 | According to the manufacture instruction |
Immunofluorescent staining | Â | Â | Â |
Goat anti-mouse CD31 antibody | R&DÂ Systems, Minnesota, USA | AF3628 | 1:100 |
Rabbit anti-mouse LYVE-1 antibody | Abcam Bioscience, Massachusetts, USA | Ab14917 | 1:100 |
Alexa Fluor 488-conjugated AffiniPure donkey anti-rabbit IgG (H + L) secondary antibody | Jackson ImmunoResearch Laboratories, Pennsylvania, USA | 711–545-152 | 1:100 |
Alexa Fluor 594-conjugated donkey anti-goat IgG (H + L) secondary antibody | Jackson ImmunoResearch Laboratories, Pennsylvania, USA | 705–585-003 | 1:100 |
hMSC culture medium | Â | Â | Â |
Basic DMEM/F-12 Medium | Gibco, Shanghai, China | C11330 | Â |
Fetal bovine serum | Gibco, Australia | 10099141C | 10% |
Basic fibroblast growth factor | PreproTech, Rocky Hill, USA | 100-18B | 10Â ng/ml |
CD4+ T cell proliferation culture medium | Â | Â | Â |
Basic RPMI 1640 Medium | Gibco, Shanghai, China | C11875 | Â |
Fetal bovine serum | Gibco, Australia | 10099141C | 10% |
Sodium pyruvate | LIFE technologist, New Mexico, USA | 11,360,070 | 1% |
HEPES buffer | LIFE technologist, New Mexico, USA | 15,630,080 | 1% |
Nonessential amino acids | LIFE technologist, New Mexico, USA | 11,140,050 | 1% |
DYNABEADS HUMAN T-ACT CD3/CD28 | Gibco, New York, USA | 11161D | According to the manufacture instruction |
Western blotting antibody | Â | Â | Â |
Thrombospondin-1(D7E5F) Rabbit mAb | Cell Signaling technology, Danvers, USA | #37,879 | 1:1000 |
Fas (C18C12) Rabbit mAb | Cell Signaling technology, Danvers, USA | #4233 | 1:1000 |
Anti-VEGF-A antibody | Abcam Bioscience, Massachusetts, USA | ab46154 | 1:1000 |
Alpha tubulin monoclonal antibody | Proteintech, Chicago, USA | 66,031–1-lg | 1:10,000 |
Anti-rabbit IgG, HRP-linked Antibody | Cell Signaling technology, Danvers, USA | #7074 | 1:2000 |
Anti-mouse IgG, HRP-linked Antibody | Cell Signaling technology, Danvers, USA | #7076 | 1:2000 |
ELISA | Â | Â | Â |
Human VEGF-C Quantikine ELISA Kit | Jianglai, Shanghai, China | JL19941 | According to the manufacture instruction |