Fig. 1

SVF treatment modified intestinal immune cells population after abdominal irradiation. A Percentage of neutrophil (CD11b⁺Ly6G⁺) population in ileum of control and at day 1, 3, 7 post-irradiation with or without SVF treatment. B Representative MPO immunostaining of the ileum at day 7 post-irradiation with or without SVF treatment. Scale bar: 100 µm. C Percentage of CD4⁺ and CD8⁺ population. D Percentage of cDC1 (CD11b⁻CD11⁺CD103⁺) and cDC2 (CD11b⁺CD11⁺CD103⁺) population in ileum of control and at day 1, 3, 7 post-irradiation with or without SVF treatment. The data are represented by mean ± SEM (for each groups n = 6) E Real-time PCR analysis of IL-1β, IL-1Rn, IFN-γ, IL-23a and IL-22 of isolated cell from ileal lamina propria. The comparative threshold cycle (Ct) method was used, and the delta Ct comparison was used to compare gene expression in cells. P values were calculated by ANOVA with Tukey correction; *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control group; #p < 0.05; ##p < 0.01; ###p < 0.001 compared with the irradiated group