Fig. 3

SVF treatment increase the pool of macrophage. A Percentage of CD11b⁺Ly6C⁻MCHII⁺ F4/80⁺ among CD45⁺ cells from ileal lamina propria of control and at day 7 post-irradiation with or without SVF treatment. The data are represented by mean ± SEM (for each groups n = 6). B Representative co-staining of F4/80 (red) and CX3CR1 (green). Dapi stained nuclei (blue). Scale bars: 50 µm. C–E Real-time PCR analysis of Inos, Arg1, CD163, MMP9, IL-10, CSF1R, CSF1 of isolated cell from ileal lamina propria. The comparative threshold cycle (Ct) method was used, and the delta Ct comparison was used to compare gene expression in cells. P values were calculated by ANOVA with Tukey correction; *p < 0.05; **p < 0.01; ***p < 0.001 compared with the control group; #p < 0.05; ##p < 0.01; ###p < 0.001 compared with the irradiated group