Fig. 4

CX3CR1 is required to mitigate irradiation damage by SVF. A Kaplan–Meier survival analysis of KO CX3CR1 mice exposed to 18 Gy abdominal irradiation with (n = 12) or without SVF treatment (n = 9). KO CX3CR1 mice show reduced survival following abdominal irradiation with 100% mortality within 9 days. No significant difference between KO CX3CR1 irradiated and KO CX3CR1 irradiated and treated SVF was observed. B IHC staining showing the expression pattern of CD24 (green), Lysozyme (red) in ileum. Dapi stained nuclei (blue). The cross section shows the co-staining of CD24 and lysozyme in the KO CX3CR1 control mice and after irradiation with or without SVF treatment. Scale bars: 50 µm. The histogram shows an increase of the Paneth cell number at day 7 after irradiation with or without SVF treatment in KO CX3CR1 mice. The data are represented by mean ± SEM (for each groups n = 4–5). C Real-time PCR analysis of IL-1β and IL-6 of ileal tissue. The comparative threshold cycle (Ct) method was used, and the delta Ct comparison was used to compare gene expression in cells. P values were calculated by ANOVA with Tukey correction; **p < 0.001 compared with the KO CX3CR1 control group; ##p < 0.001 compared with the KO CX3CR1 irradiated group; $p < 0.01 compared with the WT control group