Fig. 6

Depletion of CB11b⁺ in SVF alters SVF mitigation. A Representative visualization of GFP⁺ SVF cells (green) within ileum from irradiated-GFP-SVF-treated mice at day 1, 3, 7 (for each groups n = 6). The stem cell compartment in crypts was identified via CD44 immunostaining (red) and cell nuclei by Dapi (blue) B Strategy of CD11b gating and Flow cytometry analysis of SVF-depleted CD11b C Kaplan–Meier survival analysis of WT mice WT exposed to 18 Gy abdominal irradiation with or without SVF treatment depleted or not of CD11b, *p < 0.01 Log rank (Mantel–Cox test). D IHC co-stained with CD24 (green) and Ki67 (red) to identify the presence of proliferative cells in the intestinal stem cells compartment and IHC co-stained with CD24 (green) and Paneth cell (red) at day 7 post-irradiation of control mice and in irradiated mice with SVF depleted or not of CD11b. Dapi stained nuclei (blue). Scale bars: 50 µm. E Flow cytometry analysis of intestinal stem cells compartment (ISC). Flow cytometry analysis of EpCam population identifying the epithelial cell population and CD44, CD24, CD166, combination for ISC population at day 7 after irradiation with or without SVF treatment depleted or not of CD11b. The data are represented by mean ± SEM (for each groups n = 5–6). P values were calculated by ANOVA with Tukey correction; *p < 0.01; **p < 0.001 compared with the WT control mice; #p < 0.01; ##p < 0.001 compared with the WT irradiated mice; $$p < 0.001 compared with the WT irradiated treated SVF mice