Fig. 7

Depletion of CB11b in SVF modifies intestinal immune cells population. A neutrophil (CD11b⁺Ly6G⁺), cDC1 (CD11b⁻CD11⁺CD103⁺) and cDC2 (CD11b⁺CD11⁺CD103⁺), CD4⁺ and CD8⁺ population. B Percentage of monocytes phenotype CD11b⁺Ly6C⁺CCR2⁺, CD11b⁺Ly6C⁺MCHII⁻ CX3Cr1⁻ (P1), CD11b⁺Ly6C⁻MCHII⁺CX3Cr1^int (P2), CD11b⁺Ly6C⁻MCHII⁺CX3Cr1⁺ (P3) and CD11b⁺Ly6C⁻MCHII⁺ F4/80⁺ are analysis among CD45⁺ cells from ileal lamina propria of control and at day 7 after irradiation with or without SVF treatment depleted or not of CD11b. The data are represented by mean ± SEM (for each groups n = 6). C Real-time PCR analysis of IL-1β, IL-6 and IL-1Rn of ileal tissue. The comparative threshold cycle (Ct) method was used, and the delta Ct comparison was used to compare gene expression in cells. P values were calculated by ANOVA with Tukey correction; *p < 0.05; **p < 0.01; ***p < 0.001 compared with the WT control mice; #p < 0.05; ##p < 0.01; ###p < 0.001 compared with the WT irradiated mice; $$$p < 0.001 compared with the WT irradiated treated SVF mice