Fig. 2

Self-organization of ROs from human iPSCs on a PDMS microwell platform. A Schematic diagram of manufacturing PDMS microwell molds, including (a) design and fabrication of 3D-printed positive molds, (b) addition of PDMS biomaterials, and (c) fabrication of complementary PDMS molds. B Immunofluorescence staining images of adherent 3D ROs. (a) Ciliary margin domain was stained with RDH10 (red). (b) Neural retina domain was stained with VSX2 (green) and eye field was stained with PAX6 (red). (c, d) RPE domain was stained with ZO-1 (green), MITF (red), and PRE65 (red). Nuclei were labeled with DAPI (blue). Scale bar: 50 µm. [103] Copyright Sun et al. 2023, Biofabrication