Fig. 4
From: Bright ferritin for long-term MR imaging of human embryonic stem cells
In vitro MRI demonstrates bright contrast efficiency of ferritin-overexpressing hESCs. a T1-weighted spin echo image (T1W) and T1 map of wild type (WT) and ferritin-overexpressing (Ferritin) hESCs supplemented with 0.1 mM MnCl2 for 24 h. At baseline, WT and Ferritin hESCs displayed similar contrast levels. Upon Mn supplementation, Ferritin hESCs exhibited a T1 value that was ~ 1.7-fold lower than that of WT hESCs. b Mean T1 values ± SD of each condition calculated from T1 maps. c Intracellular Mn content measured by ICP-AES, showing that Ferritin hESCs took up ~ 1.5-fold more Mn that WT cells. Data are presented as mean ± SEM (n = 3). * P < 0.05