Fig. 4

Impact of glucose concentration on the growth and the generation of hPSC-derived pancreatic progenitors. A Schematic of experimental design B Stage-specific glucose concentration and percent glucose consumed. C Stage-specific concentration of glucose consumed and D stage-specific lactate concentration in spent media and from cells cultured in media with 5 mM, 10 mM (control) or 20 mM glucose (B-D, n = 8–10 biological replicates; two-way ANOVA with Tukey post-test). E pH measured in spent media samples (n = 5 biological replicates and the superscripts a, b, c, are significantly different from each other by one-way ANOVA with Tukey post-test). F Viability and G cell density of the respective conditions (F-G, n = 11–13 biological replicates; two-way ANOVA with Tukey post-test; orange dash line = initial seeding density). H Quantification of stage-specific glucose consumption rates and I lactate production rate (H-I, n = 6–8 biological replicates; two-way ANOVA with Tukey post-test). All box and whisker plots show individual points with median and interquartile range; bar charts show individual points with mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Spent media were sampled 24 ± 2 h from the previous media change; S1D3 = stage 1 day 3, S2D3 = stage 2 day 3, S3D2 = stage 3 day 2, S4D3 = stage 4 day 3