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Table 3 Flow cytometry antibodies

From: Metabolic switching, growth kinetics and cell yields in the scalable manufacture of stem cell-derived insulin-producing cells

Antibody

Cat #

Dilution

SOX2-PE Mouse IgG2A; Clone 245,610

IC2018P

1000X

Alexa Fluor® 647 Mouse anti-Oct-3/4 40/Oct-3 RUO

560329

50X

Goat anti-human HNF-3 beta /FoxA2 Alexa Fluor® 488-conjugated antibody

IC2400G

100X

PE Mouse anti-Human Sox17 P7-969 RUO

561591

500X

Nkx6.1 APC 

563338

50X

PE Mouse anti-PDX-1 

562161

100X

Alexa Fluor® 647 Mouse Anti-C-Peptide U8-424 RUO

565831

2500X

PE Mouse Anti-Glucagon U16-850 RUO

565860

2500X

Isotype controls

  

Ms IgG1 Kpa ItCl PE MOPC-21 100ug

554680 

 

Mouse IgG2A PE-conjugated Antibody

IC003P

 

Ms IgG1Kpa ItCl Alexa 647 MOPC-21 isotype control

557732

 

Normal Rabbit IgG Alexa Fluor® 488-conjugated Control

IC105G

 

Ms IgG1Kpa ItCl Alexa 647 MOPC-21 100Tst

557714

 

PE Mouse IgG1, κ Isotype Control 

555749

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