Fig. 3From: Induced pluripotent stem cells modulate the Wnt pathway in the bleomycin-induced model of idiopathic pulmonary fibrosisIdentification of iPSCs by real-time PCR. Real-time PCR analysis of Oct4, Sox2, Klf4, and Nanog genes in iPSCs and MEFs. Data were analyzed using the t-test method and are presented as mean ± SD (n = 6 individual wells containing about 0.5 × 10^6 cells per group at passage 3). The iPSCs expressed significantly higher levels of Oct4, Sox2, Klf4, and Nanog genes than MEFs (***P < 0.001 vs. MEFs, ****P < 0.0001 vs. MEFs), indicating the successful reprogramming of somatic cells into pluripotent stem cellsBack to article page