Fig. 3

Impact of culture passage on TF expression in UC- and DP-MSCs. a The growth ability of UC-MSCs was analyzed over passages from P3 to P10. Their PDT increased after P8. b Cell cycle profile of a representative sample depicting a G1 population, a proliferating S/G2/M population, and an apoptotic sub G1 population. c, d Analysis of five UC-MSC samples indicated decreased proliferation at P10 compared to P4 (c) and an increased apoptotic cell frequency at P10 compared to the earlier passages (d). e TF expression remained stable in the analyzed passages as indicated by flow cytometry. f–g Gene expression of the procoagulant factors TFF3 and COL1A1 (f) and the anticoagulant factors TFPI and PTGIR (g) was comparable in UC-MSCs over passages, except PTGIR tended to be decreased at higher passages compared to those in P2. h The PDT was analyzed for DP-MSCs, which demonstrated a lower growth rate at P7 and P8. i DP-MSCs tended to express a lower level of TF at higher passages. j, k Gene expression analysis of the procoagulant factors TFF3 and COL1A1 (j) and the anticoagulant factors TFPI and PTGIR (k) in DP-MSCs suggested comparable levels of these genes from P2 to P8