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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Impact of tissue factor expression and administration routes on thrombosis development induced by mesenchymal stem/stromal cell infusions: re-evaluating the dogma

Fig. 6

Impact of cell product preparation and storage on TF expression in UC- and DP-MSCs. a–c Both continuously cultured and cryopreserved/freshly thawed MSCs can be used for therapeutic purposes. Cell viability (a) and TF expression (b, c) were analyzed by flow cytometry. UC-MSCs (b) and DP-MSCs (c) under both conditions showed similar TF levels. d–g  The TF expression of continuously cultured and freshly thawed UC-MSCs (d and e, respectively) and DP-MSCs (f and g, respectively) was observed for up to 8 h of storage in NaCl. While TF was preserved on UC-MSCs of both conditions and continuously cultured DP-MSCs over the 8-h observation period, freshly thawed DP-MSCs tended to reduce its expression after eight hours. h, i The coagulation activity of continuously cultured (h) and cryopreserved/freshly thawed UC-MSCs (i) remained unchanged during an 8-h storage period

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Stem Cell Research & Therapy

ISSN: 1757-6512